CD14 Antibody (Biotin)
CD14 Antibody (Biotin)
Product Specifications
Product Name Alternative
Soluble CD14, Monocyte differentiation antigen CD14
UniProt
P08571
Reactivity
Human
Immunogen
Produced from sera of rabbits immunized with highly pure Recombinant Human sCD14. Anti-Human CD14-specific antibody was purified by affinity chromatography and then biotinylated.
Target
CD14
Clonality
Polyclonal
Conjugation
Biotin
Field of Research
Immunology & Inflammation
Purification
CD14-specific antibody was purified by affinity chromatography and then biotinylated
Concentration
Batch dependent
Form
Lyophilized
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Sandwich ELISA: To detect Human sCD14 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyt’s Polyclonal Anti-Human sCD14 as a capture antibody, allows the detection of at least 2000-4000 pg/ml of recombinant Human sCD14. Western Blot To detect Human sCD14 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human sCD14 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Tested Applications
ELISA, WB
NCBI Accession Number
P08571
Host or Source
Rabbit
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