CD274 Antibody (Biotin)
CD274 Antibody (Biotin)
Product Specifications
Product Name Alternative
Programmed Death Ligand 1, Programmed cell death 1 Ligand 1 (PDCD1L1), PD-1, B7-H1 (B7 homolog 1), CD274, SLEB2, SLE1
UniProt
Q9NZQ7
Reactivity
Human
Immunogen
Produced from sera of rabbits immunized with highly pure Recombinant Human PD-L1 Fc. Anti-Human PD-L1 Fc-specific antibody was purified by affinity chromatography and then biotinylated.
Target
CD274
Clonality
Polyclonal
Conjugation
Biotin
Field of Research
Immunology & Inflammation
Purification
PD-L1 Fc-specific antibody was purified by affinity chromatography and then biotinylated
Concentration
Batch dependent
Form
Lyophilized
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Sandwich ELISA: To detect Human PD-L1 Fc by sandwich ELISA (using 100μl/well), a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyt’s Polyclonal Anti-Human PD-L1 Fc as a capture antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human PD-L1 Fc. Western Blot To detect Human PD-L1 Fc by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human PD-L1 Fc is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Tested Applications
ELISA, WB
NCBI Accession Number
Q9NZQ7
Host or Source
Rabbit
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