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DEFB105A Antibody (Biotin)

DEFB105A Antibody (Biotin)

Product Specifications

Product Name Alternative

BD5, DEFB5, DEFB105a, Beta-Defensin 5

UniProt

Q8NG35

Reactivity

Human

Immunogen

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBD-5. Anti-Human BD-5 specific antibody was purified by affinity chromatography and then biotinylated.

Target

DEFB105A

Clonality

Polyclonal

Conjugation

Biotin

Field of Research

Immunology & Inflammation

Purification

BD-5 specific antibody was purified by affinity chromatography and then biotinylated

Concentration

Batch dependent

Form

Lyophilized

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Sandwich ELISA: To detect hBD-5 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyt’s Polyclonal Anti-Human BD-5 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hBD-5. Western Blot To detect hBD-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Tested Applications

ELISA, WB

NCBI Accession Number

Q8NG35

Host or Source

Rabbit

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