DEFB105A Antibody (Biotin)
DEFB105A Antibody (Biotin)
Product Specifications
Product Name Alternative
BD5, DEFB5, DEFB105a, Beta-Defensin 5
UniProt
Q8NG35
Reactivity
Human
Immunogen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBD-5. Anti-Human BD-5 specific antibody was purified by affinity chromatography and then biotinylated.
Target
DEFB105A
Clonality
Polyclonal
Conjugation
Biotin
Field of Research
Immunology & Inflammation
Purification
BD-5 specific antibody was purified by affinity chromatography and then biotinylated
Concentration
Batch dependent
Form
Lyophilized
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Sandwich ELISA: To detect hBD-5 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyt’s Polyclonal Anti-Human BD-5 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hBD-5. Western Blot To detect hBD-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Tested Applications
ELISA, WB
NCBI Accession Number
Q8NG35
Host or Source
Rabbit
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