Anti-MMP 9 Antibody

Mouse Monoclonal Antibody specific to MMP9 Metalloproteinase

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

MMP-9, EC 3.4.24.35, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB

Gene Name

Mmp9

NCBI Gene ID

UniProt

P50282

Cellular Locus

Secreted, extracellular space, extracellular matrix

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein corresponding to aa 1-708 (full-length) of rat MMP 9 (accession no. NP_112317).

Target Antigen

Matrix metalloproteinase-9

Target

MMP 9

Clonality

Monoclonal

Isotype

IgG2a

Type

Antibody

Applications

WB, IHC, ICC/IF, IP, FCM

Field of Research

Matrix/Metalloproteins

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration (By similarity). Could play a role in bone osteoclastic resorption (By similarity). Cleaves KiSS1 at a Gly-|-Leu bond (By similarity). Cleaves NINJ1 to generate the Secreted ninjurin-1 form (By similarity). Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide (By similarity). {UniProtKB:P14780, UniProtKB:P41245}.

Additionnal Information

Immunoblotting: use at 1-2ug/mL. Bands of ~92 and 82kDa (proenzyme and active enzyme) are detected. Immunohistochemistry: use at 1-5ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: rat brain lysate.

Storage Conditions

This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.

Specificity

This antibody recognizes human, mouse, and rat MMP 9.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.

Buffer pH

pH 7.4

Target Background

MMP 9 is involved in the breakdown of extracellular matrix in embryonic development, reproduction, wound healing, and tissue remodeling as well as in disease processes such as arthiritis and metastasis. Once synthesized, MMPs exist as latent proenzymes. Maximum MMP activity requires proteolytic cleavage of the proenzyme to generate active MMP by releasing the inhibitory propeptide domain from the full-length protein.