Anti-GluN2A/NR2A Antibody

Mouse Monoclonal Antibody specific to GluN2A/NR2A

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

GluN2A, Glutamate [NMDA] receptor subunit epsilon-1, N-methyl D-aspartate receptor subtype 2A, NMDAR2A, NR2A

Gene Name

Grin2a

NCBI Gene ID

UniProt

Q00959

Cellular Locus

Cell projection, dendritic spine, Cell membrane, Cell junction, synapse, Cell junction, synapse, postsynaptic cell membrane, Cytoplasmic vesicle membrane

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein corresponding to aa 75-325 (extracellular N-terminus) of rat GluN2A/NR2A (accession no. Q00959).

Target Antigen

Glutamate receptor ionotropic, NMDA 2A

Target

GluN2A/NR2A

Clonality

Monoclonal

Isotype

IgG2a

Type

Antibody

Applications

WB, IHC, ICC/IF

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). Sensitivity to glutamate and channel kinetics depend on the subunit composition; channels containing GRIN1 and GRIN2A have lower sensitivity to glutamate and faster deactivation kinetics than channels formed by GRIN1 and GRIN2B (PubMed:28384476). Contributes to the slow phase of excitatory postsynaptic current, long-term synaptic potentiation, and learning (By similarity). {UniProtKB:P35436, PubMed:1350383, PubMed:16281028, PubMed:23625947, PubMed:24462099, PubMed:26679993, PubMed:27618671, PubMed:27916457, PubMed:28384476, PubMed:28468946, PubMed:28760974, PubMed:8428958}.

Additionnal Information

Immunoblotting: use at 1ug/mL. A band of ~170kDa is detected. <br><br>Positive control: Rat brain lysate. <br><br>These are recommended concentrations. User should determine optimal concentrations for their application.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, and rat GluN2A/NR2A. It does not cross- react with NR2B.

Formulation

PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.

Buffer pH

pH 7.4

Target Background

N-methyl-D-aspartate receptors are heteromeric protein complexes, and three families of NMDAR subunits have been identified: NR1, NR2 and NR3. The subunit composition determines the pharmacological and physiological properties of the NMDARs. NR2A is gradually increased in adult CNS but is absent in embryonic CNS. Electro- physiological data suggest that NR2A- containing NMDARs tend to be localized synaptically, whereas NR2B-containing receptors tend to be localized peri- synaptically or extrasynaptically. In the spinal cord, the predominant subunits of NMDARs include NR2A at synaptic sites and NR2B at extrasynaptic sites.