EIF2B5 Recombinant Rabbit Monoclonal Antibody [JE34-98]
Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on eukaryotic initiation factor 2 (eIF2) gamma subunit. Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl-initiator methionine tRNA available to the ribosome and consequently global translation is repressed. Activated by the chemical integrated stress response (ISR) inhibitor ISRIB which stimulates guanine nucleotide exchange factor activity for both phosphorylated and unphosphorylated eIF2.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
EIF2B5, EIF2BE
Swiss Prot
Q13144 Human
Cellular Locus
Cytoplasm, cytosol.
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Synthetic peptide within Human EIF2B5 aa 1-50 / 721.
Isotype
IgG
Clone
JE34-98
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Cell
Positive Control
HeLa cell lysate, 293T cell lysate, Jurkat cell lysate, HeLa.
Concentration
1 mg/mL
Dilution
WB: 1:1,000 ;IF-Cell: 1:100
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 80 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
Yes
Frequently Asked Questions
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