THPO Antibody (Biotin)
THPO Antibody (Biotin)
Product Specifications
Product Name Alternative
Thrombopoietin, Megakaryocyte colony-stimulating factor, c-MPL Ligand, MGDF
UniProt
P40225
Reactivity
Human
Immunogen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTPO. Anti-Human TPO specific antibody was purified by affinity chromatography and then biotinylated.
Target
THPO
Clonality
Polyclonal
Conjugation
Biotin
Field of Research
Cell Biology
Purification
TPO specific antibody was purified by affinity chromatography and then biotinylated
Concentration
Batch dependent
Form
Lyophilized
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Sandwich ELISA: To detect hTPO by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyt’s Polyclonal Anti-Human TPO as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hTPO. Western Blot To detect hTPO by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTPO is 1.5 - 3.0 ng/lane, under either reducing or non-reducing condition
Tested Applications
ELISA, WB
NCBI Accession Number
P40225
Host or Source
Rabbit
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