Terminal Transferase Buffer Solution

<strong>Terminal Transferase Buffer</strong>_x000D_ <table width="1028">_x000D_ <tbody>_x000D_ <tr>_x000D_ <td width="285">Catalog number:</td>_x000D_ <td width="743">B2010101</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Lot number:</td>_x000D_ <td>Batch Dependent</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Expiration date:</td>_x000D_ <td>Batch Dependent</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Volume/Weight</td>_x000D_ <td>4 x 1 mL</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>pH</td>_x000D_ <td>6.8</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Supplied as</td>_x000D_ <td>Ready-to-use solution</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Appearance</td>_x000D_ <td>Clear solution</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Applications</td>_x000D_ <td>Optimized buffer for use with Terminal Deoxynucleotidyl Transferase, an enzyme which catalyzes the processive addition of dNTPs to the 3´-OH end of a single-stranded DNA primer.</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Shelf-Life</td>_x000D_ <td>1 year from date of manufacture</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Storage:</td>_x000D_ <td>Keep at 2-8°C</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Keywords:</td>_x000D_ <td>Terminal Transferase Buffer, TT buffer</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Grade</td>_x000D_ <td>Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered on a 0.22 um.</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>References</td>_x000D_ <td width="743">1: Liu K, Zhao E, Ilyas G, Lalazar G, Lin Y, Haseeb M, Tanaka KE, Czaja MJ._x000D_ Impaired macrophage autophagy increases the immune response in obese mice by_x000D_ promoting proinflammatory macrophage polarization. Autophagy. 2015;11(2):271-84._x000D_ _x000D_ _x000D_ 2: Tang F, Barbacioru C, Nordman E, Li B, Xu N, Bashkirov VI, Lao K, Surani MA._x000D_ RNA-Seq analysis to capture the transcriptome landscape of a single cell. Nat_x000D_ Protoc. 2010 Mar;5(3):516-35._x000D_ _x000D_ 3: Gazit N, Vertkin I, Shapira I, Helm M, Slomowitz E, Sheiba M, Mor Y, Rizzoli_x000D_ S, Slutsky I. IGF-1 Receptor Differentially Regulates Spontaneous and Evoked_x000D_ Transmission via Mitochondria at Hippocampal Synapses. Neuron. 2016 Feb_x000D_ 3;89(3):583-97._x000D_ _x000D_ 4: Deng G, Wu R. An improved procedure for utilizing terminal transferase to add_x000D_ homopolymers to the 3' termini of DNA. Nucleic Acids Res. 1981 Aug_x000D_ 25;9(16):4173-88._x000D_ _x000D_ 5: Zhang C, Dai P, Spokoyny AM, Pentelute BL. Enzyme-catalyzed macrocyclization_x000D_ of long unprotected peptides. Org Lett. 2014 Jul 18;16(14):3652-5._x000D_ _x000D_ 6: Yamashita S. Heat-induced antigen retrieval: mechanisms and application to_x000D_ histochemistry. Prog Histochem Cytochem. 2007;41(3):141-200._x000D_ _x000D_ 7: Niyomrattanakit P, Wan KF, Chung KY, Abas SN, Seh CC, Dong H, Lim CC, Chao_x000D_ AT, Lee CB, Nilar S, Lescar J, Shi PY, Beer D, Lim SP. Stabilization of dengue_x000D_ virus polymerase in de novo initiation assay provides advantages for compound_x000D_ screening. Antiviral Res. 2015 Jul;119:36-46._x000D_ _x000D_ 8: Ma LN, Li LD, Li SC, Hao XM, Zhang JY, He P, Li YK. Allicin improves cardiac_x000D_ function by protecting against apoptosis in rat model of myocardial infarction._x000D_ Chin J Integr Med. 2017 Aug;23(8):589-597._x000D_ _x000D_ 9: Zheng J, Liu Z, Li W, Tang J, Zhang D, Tang X. Lithium posttreatment confers_x000D_ neuroprotection through glycogen synthase kinase-3β inhibition in intracerebral_x000D_ hemorrhage rats. <a href="https://pubmed.ncbi.nlm.nih.gov/27739937/">J Neurosurg.</a> 2017 Oct;127(4):716-724.</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Related Products</td>_x000D_ <td><a href="https://moleculardepot.com/product-category/buffers/">Biochemical Buffers and Solutions</a></td>_x000D_ </tr>_x000D_ </tbody>_x000D_ </table>_x000D_ &nbsp;