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SARS-CoV-2 S2 protein (A701V), His Tag

SARS-CoV-2 S2 protein (A701V), His Tag (S2N-C52Hc) is expressed from human 293 cells (HEK293) . It contains AA Ser 686 - Pro 1213 (Accession # QHD43416.1) . The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P/ A892P/ A899P/ A942P/ K986P/ V987P) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein. The A701V mutation was identified in the SARS-CoV-2 Beta variant (Pango lineage: B.1.351; other names: 20H/501Y.V2) .

Product Specifications

Background

It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 59.8 kDa. The protein migrates as 70-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

QHD43416.1

Expression Region

Ser 686 - Pro 1213

Host

HEK293

Target

Spike S2

Conjugation

Unconjugated

Tag

C-10xHis

Source

SARS-CoV-2

Stability

● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized SARS-CoV-2 S2 protein (A701V), His Tag (Cat. No. S2N-C52Hc) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike S2 protein Antibody, Human IgG4 (Cat. No. S2N-S86) with a linear range of 0.2-2 ng/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

59.8 kDa

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

500ug*2

Product Datasheet

Https://www.acrobiosystems.com/static/main/products/pdf/ds/S2N-C52Hc.pdf

Species

SARS-CoV-2

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