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Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*15:01&MBP (ENPVVHFFKNIVTPR) Complex Protein (Monomer, MALS verified)

Selected Variant

HLM-H82Ea-25ug

Size: 25 µg

Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*15:01&MBP (ENPVVHFFKNIVTPR) Complex Protein (HLM-H82Ea) is expressed from human 293 cells (HEK293) . It contains AA Ile 26 - Glu 216 (HLA-DRA1*01:01) & Gly 30 - Lys 227 (HLA-DRB1*15:01) & ENPVVHFFKNIVTPR peptide (Accession # CAI2388006.1 (HLA-DRA1*01:01) & CAI2388008.1 (HLA-DRB1*15:01) & ENPVVHFFKNIVTPR) .

Product Specifications

Specifications

Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*15:01&MBP (ENPVVHFFKNIVTPR) Complex Protein is produced by co-expression of HLA-DRA1 and HLA-DRB1 loaded with MBP peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 29.8 kDa and 29.2 kDa. The protein migrates as 37-40 kDa and 32-37 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

CAI2388006.1 (HLA-DRA1*01:01) & CAI2388008.1 (HLA-DRB1*15:01) & ENPVVHFFKNIVTPR

Expression Region

Ile 26 - Glu 216 (HLA-DRA1*01:01) & Gly 30 - Lys 227 (HLA-DRB1*15:01) & ENPVVHFFKNIVTPR peptide

Host

HEK293

Target

HLA-DRA1*0101 & HLA-DRB1*1501 & MBP (ENPVVHFFKNIVTPR)

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*15:01&MBP (ENPVVHFFKNIVTPR) Complex Protein (Cat. No. HLM-H82Ea) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA-DR Antibody, Human IgG1 with a linear range of 0.06-2 ng/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

29.8 kDa and 29.2 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

25ug*1

Host or Source

HEK293

Species

Human

Protein ID

CAI2388006.1 (HLA-DRA1*01:01) & CAI2388008.1 (HLA-DRB1*15:01) & ENPVVHFFKNIVTPR

Preservative

Trehalose

Price

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