SARS-CoV-2 Spike NTD Protein (T95I, G142D, E154K), His Tag (MALS verified)

SARS-CoV-2 Spike NTD (T95I, G142D, E154K), His Tag (S1D-C52Hf) is expressed from human 293 cells (HEK293). It contains AA Ser 13 - Leu 303 (Accession # QHD43416.1 (T95I, G142D, E154K)). The mutations (T95I, G142D, E154K) were identified in the SARS-CoV-2 Kappa variant (Pango lineage: B.1.617.1; other names: 21A/S:154K).

Product Specifications

Background

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 35.0 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Ser 13 - Leu 303

Host

HEK 293

Target

Spike NTD

Conjugation

Unconjugated

Tag

C-10×His

Source

SARS-CoV-2

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized SARS-CoV-2 Spike NTD (T95I, G142D, E154K), His Tag (Cat. No. S1D-C52Hf) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike NTD Antibody, Chimeric mAb (Cat. No. SPD-M121) with a linear range of 0.1-1 ng/mL (QC tested).