Anti-Ankyrin-G Antibody

Mouse Monoclonal Antibody specific to Ankyrin-G

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

ANK-3, Ankyrin-G

Gene Name

ANK3

NCBI Gene ID

UniProt

Q12955

Cellular Locus

Cytoplasm, cytoskeleton, Cell projection, axon, Cell membrane, sarcolemma, Cell junction, synapse, postsynaptic cell membrane, Lysosome, Cell membrane, sarcolemma, T-tubule

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein corresponding to ~1,000 C-terminal amino acids of human Ankyrin G (accession no. NP_066267.2).

Target Antigen

Ankyrin-3

Target

Ankyrin-G

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, ICC/IF

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

In skeletal muscle, required for costamere localization of DMD and betaDAG1 (By similarity). Membrane-cytoskeleton linker. May participate in the maintenance/targeting of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments. Regulates KCNA1 channel activity in function of dietary Mg(2+) levels, and thereby contributes to the regulation of renal Mg(2+) reabsorption (PubMed:23903368). {PubMed:17974005}.; [Isoform 5]: May be part of a Golgi-specific membrane cytoskeleton in association with beta-spectrin. {PubMed:17974005}.

Additionnal Information

Immunoblotting: use at 1-2ug/mL. A band of >200kDa is detected. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: rat brain membranes.

Storage Conditions

This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.

Specificity

This antibody recognizes human, mouse, and rat Ankyrin-G.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.

Buffer pH

pH 7.4

Target Background

Ankyrins are a family of adaptor proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane skeleton. Ankyrins have binding sites for the beta subunit of spectrin and for at least 12 families of integral membrane proteins. This linkage is required to maintain the integrity of the plasma membranes and to anchor specific ion channels, ion exchangers, and ion transporters in the plasma membrane.