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Anti-P73 (Phospho-Tyr99) Antibody

Rabbit Polyclonal Antibody specific to P73 (Phospho-Tyr99)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-P73 (Phospho-Tyr99) Rabbit Polyclonal Antibody

Host

Rabbit

Reactivity

Human

Immunogen

Peptide sequence that includes phosphorylation site of tyrosine 99 (S-P-Y(p)-A-Q) derived from human p73.

Target

P73 (Phospho-Tyr99)

Clonality

Polyclonal

Type

Antibody

Applications

WB

Field of Research

Phosphospecific Antibodies

Purification Method

Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~80kDa is detected. <br><br>These are recommended working dilutions. <br><br>End user should determine optimal dilutions for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody detects endogenous human p73 only when phosphorylated at tyrosine 99.

Formulation

PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Buffer pH

pH 7.4

Target Background

Tumor protein p73 is a member of the p53 family of transcription factors involved in cellular responses to stress and development. The family members include p53, p63, and p73 and have high sequence similarity to one another which allows p63 and p73 to transactivate p53- responsive genes causing cell cycle arrest and apoptosis. The family members can interact with each other in many ways involving direct or indirect protein interactions, resulting in regulation of the same target gene promoters or regulation of each other's promoters. The p73 protein is expressed at very low levels in normal tissues and is differentially expressed in a number of tumors.

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