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NFF-3

NFF-3, the peptide, is a selective MMP substrate. NFF-3 selectively binds to MMP-3 and MMP-10 to be hydrolyzed. NFF-3 is also cleaved by trypsin, hepatocyte growth factor activator, and factor Xa. Label NFF-3 with a CyDye pair, Cy3/Cy5Q, can produce fluorescence in cell assays to detect cell activity[1].

Product Specifications

CAS Number

[158584-09-9]

UNSPSC

12171500

Target

MMP

Type

Dye Reagents

Related Pathways

Metabolic Enzyme/Protease

Applications

Metabolism-protein/nucleotide metabolism

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/nff-3.html

Solubility

10 mM in DMSO

Smiles

O=[N+]([O-])C(C=C(C=C1)[N+]([O-])=O)=C1NCCCC[C@@H](C(N)=O)NC([C@H](CCCNC(N)=N)NC([C@@H](NC([C@H](CCC)NC([C@H](CCC(O)=O)NC([C@H](C(C)C)NC([C@H]2N(CCC2)C([C@H](CCCCN)NC([C@H]3N(CCC3)C([C@H](CCCNC(N)=N)NC(CC(C4=CC=C(OC)C=C4O5)=CC5=O)=O)=O)=O)=O)=O)=O)=O)=O)CC6=CNC7=CC=CC=C67)=O)=O

Molecular Formula

C78H110N22O20

Molecular Weight

1675.84

References & Citations

[1]Giricz O, et al. Comparison of metalloproteinase protein and activity profiling. Anal Biochem. 2011 Feb 1;409 (1) :37-45.

Shipping Conditions

Room temperature

Scientific Category

Dye Reagents

Clinical Information

No Development Reported

Isoform

MMP-10; MMP-3

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