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Rabbit anti Human Actin, cytoplasmic 1 (NT)

Product Specifications

Background

Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, b-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

UniProt

P60709

Host

Rabbit

Species Reactivity

Human

Isotype

Ig

Conjugation

Unconjugated

Type

Polyclonal Antibody

Applications

WB

Field of Research

Ancillary Reagents

Purification Method

Ammonium Sulfate Precipitation

Assay Principle

Antibody can be used for Western blotting (1-5 µg/mL). Optimal concentration should be evaluated by serial dilutions.

Stability

See expiration date on vial

Concentration

See vial for Concentration

Form

Provided as solution in phosphate buffered saline with 0,08% sodium azide

Precautions

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

References & Citations

1. Ponte P., Ng S.Y., Engel J., Gunning P., Kedes L., Evolutionary conservation in the untranslated regions of actin mRNAs: DNA sequence of a Human beta-actin cDNA. Nucleic Acids Res. 12:1687-1696(1984) 2. Nakajima-Iijima S., Hamada H., Reddy P., Kakunaga T.,Molecular structure of the Human cytoplasmic beta-actin gene: interspecies homology of sequences in the introns. Proc. Natl. Acad. Sci. U.S.A. 82:6133-6137(1985) 3. Procaccio V., Salazar G., Ono S., Styers M.L., Gearing M., Davila A., Jimenez R., Juncos J., Gutekunst C.-A., Meroni G., Fontanella B., Sontag E., Sontag J.-M., Faundez V., Wainer B.H. A mutation of beta -actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia. Am. J. Hum. Genet. 78:947-960(2006) [PubMed: 16685646] [Abstract]

Shipping Conditions

Ambient Temperature, freeze upon arrival

Storage Conditions

Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles

Functional Analysis

WB

CAS Number

9007-83-4

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