Anti-Protocadherin γ (pan) Antibody

Mouse Monoclonal Antibody specific to Protocadherin γ (pan)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-Protocadherin γ (pan) Mouse Monoclonal Antibody

Gene Name

Pcdhga1

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=Pcdhga1">Pcdhga1</a>

UniProt

Q91XZ0

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein corresponding to aa 808-931 (C-terminal cytoplasmic constant domain) of mouse PCDHGA1. This sequence is present in all 22 PCDHG proteins.

Target Antigen

Protocadherin γ A1

Target

Protocadherin γ (pan)

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, ICC/IF

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Immunoblotting: use at 1-2ug/mL. A band of ~100kDa is detected. Immunofluorescence: use at 10ug/mL. These are recommended concentrations. Endusers should determine optimal concentrations for their application.

Storage Conditions

This product is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, and rat PCDHG-A, B, and C proteins

Formulation

PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.

Buffer pH

pH 7.4

Target Background

The protocadherin gamma gene cluster is one of three related clusters tandemly linked on chromosome five. The gamma gene cluster includes 22 genes divided into 3 subfamilies: subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region (which includes 6 cadherin ectodomains and a trans-membrane region). The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins are believed to play a critical role in the establishment and function of specific cell-cell connections in the brain.