Anti-DcR2 Antibody

Rabbit Polyclonal Antibody specific to DcR2

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Decoy receptor 2, DcR2, TNF-related apoptosis-inducing ligand receptor 4, TRAIL receptor 4, TRAIL-R4, TRAIL receptor with a truncated death domain, CD antigen CD264

Gene Name

TNFRSF10D

Gene ID

8793

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=TNFRSF10D">TNFRSF10D</a>

UniProt

Q9UBN6

Accession Number

NP_003831

Cellular Locus

Membrane, Single-pass type I membrane protein

Host

Rabbit

Reactivity

Human

Immunogen

Peptide corresponding to aa 249-263 of human DcR2 precursor (accession no. Q9UBN6).

Target Antigen

Tumor necrosis factor receptor superfamily member 10D

Target

DcR2

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, ICC, IHC-P, IF

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Receptor for the cytotoxic ligand TRAIL (PubMed:9430226). Contains a truncated death domain and hence is not capable of inducing apoptosis but protects against TRAIL-mediated apoptosis (PubMed:9537512). Reports are contradictory with regards to its ability to induce the NF-kappa-B pathway. According to PubMed:9382840, it cannot but according to PubMed:9430226, it can induce the NF-kappa-B pathway (PubMed:9382840, PubMed:9430226). {PubMed:9382840, PubMed:9430226, PubMed:9537512}.

Additionnal Information

Immunoblotting: use at 1ug/mL. Immunocytochemistry: use at 10ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: Whole cell lysate from HeLa cells.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes full-length DcR2 (34kDa).

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

TRAIL/Apo2L is a new member of the TNF family and induces apoptosis in a variety of tumor cell lines. DR4 and DR5 are the recently identified functional receptors for TRAIL, and DcR1/TRID is a â€œdecoy receptorâ€. Another member of the TRAIL receptor family was more recently identified and designated DcR2, TRAIL- R4, or TRUNDD. DcR2 has an extracellular TRAIL-binding domain but lacks an intracellular death domain and does not induce apoptosis. Like DR4 and DR5, DcR2 transcript is widely expressed in normal human tissues. Overexpression of DcR2 attenuates TRAIL- induced apoptosis.