Phospho-Vimentin (S39) Recombinant Rabbit Monoclonal Antibody [JE43-26]

Vimentin is a structural protein that in humans is encoded by the VIM gene. Its name comes from the Latin vimentum which refers to an array of flexible rods. IF proteins are found in all animal cells as well as bacteria. Intermediate filaments, along with tubulin-based microtubules and actin-based microfilaments, comprises the cytoskeleton. All IF proteins are expressed in a highly developmentally-regulated fashion; vimentin is the major cytoskeletal component of mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression.

Product Specifications

CAS Number

9000-83-3

Product Name Alternative

CTRCT30 antibody Epididymis luminal protein 113 antibody FLJ36605 antibody HEL113 antibody VIM antibody VIME_HUMAN antibody Vimentin antibody

Abbreviation

VIM

Swiss Prot

P08670 Human, P20152 Mouse, P31000 Rat

Cellular Locus

Cytoplasm, cytoskeleton, Nucleus matrix, Cell membrane.

Host

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser39 of human vimentin protein.

Isotype

IgG

Clone

JE43-26

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell

Positive Control

HeLa treated with 100nM Calyculin A for 30 minutes whole cell lysate, HeLa treated with 100nM Calyculin A for 30 minutes, NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate, C6 treated with 100nM Calyculin A for 30 minutes cell lysate, NIH/3T3 treated with 100nM Calyculin A for 30 minutes.