Anti-T7 Antibody
Rabbit Polyclonal Antibody specific to T7
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Anti-T7 Rabbit Polyclonal Antibody
Host
Rabbit
Reactivity
T7
Immunogen
T7 (MASMTGGQQMG) conjugated to KLH.
Target
T7
Clonality
Polyclonal
Type
Antibody
Applications
WB, IP, ICC, ELISA
Field of Research
Epitope Tags
Purification Method
Purified by peptide immuno-affinity chromatography
Concentration
1.0 mg/mL
Dilution
Dilute in PBS or medium that is identical to that used in the assay system.
Format
Purified
Form
Liquid
Buffer
Phosphate Buffered Saline
Additionnal Information
Immunoblotting: use at a dilution of 1:1,000- 1:20,000.
<br><br>Immunocytochemistry: use at a dilution of 1:100-1:400.
<br><br>Immunoprecipitation: use 1-4ug/mg lysate.
<br><br>ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100-1:500
<br><br>These are recommended dilutions.
<br><br>Endusers should determine optimal dilutions for their applications.
Storage Conditions
This antibody is stable for at least one (1) year at 2-8°C.
Specificity
Antibody was affinity-purified on the peptide.
Formulation
PBS, pH 7.2, 0.09% sodium azide.
Buffer pH
pH 7.2
Target Background
The T7 tag is an 11 amino acid peptide (MASMTGGQQMG) derived from the leader sequence of T7 bacteriophage gene10 which encodes a T7 major capsid protein. The T7 tag serves as a tag in many expression vectors, and antibodies specific for the T7 tag are useful for identifying and isolating T7-tagged proteins.
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