Phenylalanine (Phe) Colorimetric Assay Kit
Product Specifications
Type
Colorimetric method
Field of Research
Liver and Renal Function; Amino Acids and Proteins
Assay Principle
Phenylalanine (Phe), chemically known as 2-amino-3-phenylpropionic acid, is an essential amino acid involved in a variety of biosynthetic processes. In the body, most Phe is oxidized to tyrosine by phenylalanine hydroxylase, and together with tyrosine, it synthefies important neurotransmitters and hormones, and participates in glucose metabolism and fat metabolism. Phenylalanine is catalyzed by enzyme , and the chromogenic agent has an absorption peak at 450 nm. The Phe content of the sample can be calculated by measuring the OD value of the chromogenic substance at 450 nm.
Assay Performance Time
50 min
Sample Type
Serum; Plasma; Urine; Animal and plant tissue; Cell; Cell culture supernatant
Detection Range
4.25-500.00 μmol/L
Sensitivity
4.25 μmol/L
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at -20°C for 12 months with shading light.
Inter CV
2.4-4.0%
Intra CV
0.9-1.6%
Detection Instrument
Microplate reader
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