ULK3 Rabbit Polyclonal Antibody
Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Abbreviation
ULK3; Serine/threonine-protein kinase ULK3; Unc-51-like kinase 3
Swiss Prot
Q6PHR2 Human, Q3U3Q1 Mouse, D3ZHP7 Rat
Cellular Locus
Cytoplasm.
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Recombinant protein within human ULK3 aa 1-472 / 472.
Isotype
IgG
Conjugation
Non-conjugated
Type
Rabbit polyclonal Antibody
Applications
WB, FC
Positive Control
293T cell lysate, HeLa cell lysate, Jurkat cell lysate, mouse brain tissue lysate, rat brain tissue lysate, HeLa.
Concentration
1 mg/mL
Dilution
WB: 1:2,000 ;FC: 1:1,000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 53 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
No
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