Human FGF-21 Protein, His tag (Animal-Free)

Human FGF-21 Protein, His tag (Animal-Free), expressed in E. coli

Product Specifications

Background

Fibroblast Growth Factors-21 (FGF-21) is a 22.3 kDa member of the fibroblast Growth Factors with 209 amino acid residues. FGF-21 is expressed from liver and cardiomyocytes. FGF-21 is a key protein that regulates important metabolic pathways, and modulates cellular function, metabolism, and senescence. It can stimulate glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1 /GLUT1 expression.

Product Name Alternative

FGFL

Accession Number

Q9NSA1

Tag

FGF-21

Type

Cytokines and proteins

Applications

His tag

Purity

> 98% as determined by SDS-PAGE.

Activity

Measure by its ability to induce proliferation in BaF3 cells transfected with human FGFRIIIc.The ED₅₀ for this effect is <0.4 μg/mL.

Molecular Weight

21.35 kDa

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.

Shipping Conditions

The product is shipped with blue ice.

Storage Conditions

Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA) . Please prevent freeze-thaw cycles

Protein Length

The protein has a calculated MW of 21.35 kDa. The protein migrates as 25 kDa under reducing condition (SDS-PAGE analysis) .

Formulation

Lyophilized from sterile PBS, pH8.0

Applications Notes

Human

Other Product Names

Human FGF-21 Protein, His tag (Animal-Free), FGF-21, FGFL

Recommended Usage

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.