3mC Recombinant Rabbit Monoclonal Antibody [PSH03-21]
Methylation of DNA can occur non-enzymatically at the nitrogen-three of the cytosine base through spontaneous exposure to endogenous S-adenosyl methionine (SAM). The resulting 3-methylcytosine (3-mC) is mutagenic and must be repaired, which occurs in humans through the base excision repair (BER) or dealkylation via human homologues of the E. coli AlkB protein. 3-methylcytosine is present in human cell lines and increased levels of 3-mC impair proliferation.
Product Specifications
CAS Number
9000-83-3
Host
Rabbit
Species Reactivity
Universal
Immunogen
3-mc-OVA
Isotype
IgG
Clone
PSH03-21
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
IF-Cell, Dot Blot
Positive Control
U-2 OS cells treated with 4mM Methyl Methanesulfonate for 1 hour.
Concentration
1 mg/mL
Dilution
IF-Cell: 1:100 ;Dot Blot: 1:2,000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
Yes
Frequently Asked Questions
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