Mouse anti Ovarian Cancer associated antigen

A central concept in tumor immunology is that malignancies bear tumor associated antigens, which are not or to a much lesser degree found in normal tissues. These antigens can be used as distinctive markers in diagnosis and therapy. As a consequence, monoclonal antibodies to such antigens may represent useful tools for histopathology, immunoscintigraphy and for immunotherapy.

OVTL16 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with cyst fluid, obtained from a patient with a poorly differentiated Human ovarian carcinoma.

OVTL16 is suitable for immunohistochemistry on frozen tissues and for staining with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent. Optimal antibody dilution should be determined by titration

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

Boerman, O., Makkink, K., Massuger, L., Thomas, C., Kenemans, P., Hanselaar, T., and Poels, L. (1989). Monoclonal antibodies anti ovarian carcinoma-associated antigens, raised by immunization with cyst fluids, Anticancer Res 9, 551-8 _x000D_ Boerman, O., Massuger, L., Makkink, K., Thomas, C., Kenemans, P., and Poels, L. (1990). Comparative in vitro binding characteristics and biodistribution in tumor-bearing athymic mice of anti-ovarian carcinoma monoclonal antibodies. Anticancer Res 10, 1289-1295. _x000D_ van Niekerk, C. C., Ramaekers, F. C., Hanselaar, A. G., Aldeweireldt, J., and Poels, L. G. (1993). Changes in expression of differentiation markers between normal ovarian cells and derived tumors, Am J Pathol 142, 157-77. _x000D_ Slobbe, R., Poels, L., ten Dam, G., Boerman, O., Nieland, L., Leunissen, J., Ramaekers, F., and van Eys, G. (1994). Analysis of idiotope structure of ovarian cancer antibodies: recognition of the same epitope by two monoclonal antibodies differing mainly in their heavy chain variable sequences, Clin Exp Immunol 98, 95-103. _x000D_ van Niekerk, C. C., Vooijs, P., Casparie-van Velsen, I. J., and Poels, L. G. (1997). Differentiation margins of ovarian tumor pathology: first incidences of epithelial ovarian tumors monitored by marker antibodies. Cancer Detect Prev 21, 247-257.

The antibody is shipped at ambient temperature and may be stored at +4°C; For prolonged storage prepare appropriate aliquots and store at or below -20°C; Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2); Repeated thawing and freezing should be avoided; Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day; If a slight precipitation occurs upon storage, this should be removed by centrifugation; It will not affect the performance or the concentration of the product