Biotin-azide

Biotin-azide (N- (3-Azidopropyl) biotinamide) is a form of biotin with a terminal azide group. Biotin-azide can be used to prepare various biotinylated conjugates via Click Chemistry[1][2]. Biotin-azide is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.

Product Specifications

CAS Number

[908007-17-0]

Product Name Alternative

N- (3-Azidopropyl) biotinamide

UNSPSC

12352200

Target

Biochemical Assay Reagents

Type

Biochemical Assay Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/biotin-azide.html

Purity

99.84

Solubility

DMSO : 100 mg/mL (ultrasonic) |H2O : 4 mg/mL (ultrasonic)

Smiles

O=C(NCCCN=[N+]=[N-])CCCC[C@@H]1SC[C@]([C@]1([H])N2)([H])NC2=O

Molecular Formula

C13H22N6O2S

Molecular Weight

326.42

References & Citations

[1]Bruckman MA, et al. Tobacco mosaic virus-based protein nanoparticles and nanorods for chemotherapy delivery targeting breast cancer. J Control Release. 2016;231:103‐113.|[2]Kim HY, et al. An azido-biotin reagent for use in the isolation of protein adducts of lipid-derived electrophiles by streptavidin catch and photorelease. Mol Cell Proteomics. 2009;8 (9) :2080‐2089.|[3]Joiner CM, et al. A Bifunctional Amino Acid Enables Both Covalent Chemical Capture and Isolation of in Vivo Protein-Protein Interactions. Chembiochem. 2017 Jan 17;18 (2) :181-184.