HEPES

HEPES, a nonvolatile zwitterionic chemical buffering agent, is broadly applied in cell culture. HEPES is effective at pH 6.8 to 8.2. HEPES is also a potent inducer of lysosome biogenesis[1][2][3].

Product Specifications

CAS Number

[7365-45-9]

UNSPSC

12352200

Hazard Statement

H302, H315, H319, H335

Target

Biochemical Assay Reagents

Type

Biochemical Assay Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/hepes.html

Purity

99.94

Solubility

H2O : 250 mg/mL (ultrasonic)

Smiles

O=S(CCN1CCN(CCO)CC1)(O)=O

Molecular Formula

C8H18N2O4S

Molecular Weight

238.30

Precautions

H302, H315, H319, H335

References & Citations

[1]Suzuki T, et al. Nonvolatile buffer coating of titanium to prevent its biological aging and for drug delivery. Biomaterials. 2010;31 (18) :4818-4828.|[2]Sledź P, et al. An experimental charge density of HEPES. Acta Crystallogr B. 2010;66 (Pt 4) :482-492.|[3]Tol MJ, et al. HEPES activates a MiT/TFE-dependent lysosomal-autophagic gene network in cultured cells: A call for caution. Autophagy. 2018;14 (3) :437-449.