Puromycin aminonucleoside

Puromycin aminonucleoside (NSC 3056) is the aminonucleoside portion of the antibiotic puromycin, and used in nephrosis animal models[1]. Puromycin aminonucleoside induces apoptosis[2]. Puromycin aminonucleoside is a reversible inhibitor of dipeptidyl peptidase II and cytosol alanyl aminopeptidase[3]. Puromycin aminonucleoside induces secretion of cell migrasome[4].

Product Specifications

CAS Number

[58-60-6]

Product Name Alternative

NSC 3056

UNSPSC

12352005

Hazard Statement

H302, H315, H319, H335

Target

Aminopeptidase; Antibiotic; Apoptosis; Bacterial; Dipeptidyl Peptidase

Type

Reference compound

Related Pathways

Anti-infection; Apoptosis; Metabolic Enzyme/Protease

Applications

Cancer-Kinase/protease

Field of Research

Cancer

Assay Protocol

https://www.medchemexpress.com/Puromycin-aminonucleoside.html

Purity

99.86

Solubility

DMSO : 25 mg/mL (ultrasonic; warming; heat to 60°C) |H2O : 40 mg/mL (ultrasonic; warming; heat to 50°C)

Smiles

N[C@H]1[C@@H](O)[C@H](N(C=N2)C3=C2C(N(C)C)=NC=N3)O[C@@H]1CO

Molecular Formula

C12H18N6O3

Molecular Weight

294.31

Precautions

H302, H315, H319, H335

References & Citations

[1]Wada T, et al. Prevents podocyte apoptosis induced by puromycin aminonucleoside: role of p53 and Bcl-2-related family proteins. J Am Soc Nephrol. 2005 Sep;16 (9) :2615-25.|[2]Xia L, et al. Podocyte-specific expression of organic cation transporter PMAT: implication in puromycin aminonucleosidenephrotoxicity. Am J Physiol Renal Physiol. 2009 Jun;296 (6) :F1307-13.|[3]Kawakami H, et al. Dynamics of absolute amount of nephrin in a single podocyte in puromycin aminonucleoside nephrosis rats calculated by quantitative glomerular proteomics approach with selected reaction monitoring mode. Nephrol Dial Transplant. 2012 Apr; |[4]Nosaka K, et al. An adenosine deaminase inhibitor prevents puromycin aminonucleoside nephrotoxicity. Free Radic Biol Med 1997 ;22 (4) : 597-605.|[5]Lacalle RA, et al. Cloning of the complete biosynthetic gene cluster for an aminonucleoside antibiotic, puromycin, and its regulated expression in heterologous hosts. EMBO J. 1992 Feb;11 (2) :785-92.|[6]Gong W, et al. Estrogen-related receptor-α mediates puromycin aminonucleoside-induced mesangial cell apoptosis and inflammatory injury. Am J Physiol Renal Physiol. 2019 May 1;316 (5) :F906-F913.|[7]Ying Liu, et al. Podocyte-Released Migrasomes in Urine Serve as an Indicator for Early Podocyte Injury. Kidney Dis (Basel) . 2020 Nov;6 (6) :422-433.