Anti-E2F-1 Antibody

Mouse Monoclonal Antibody specific to E2F-1

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

E2F-1, PBR3, Retinoblastoma-associated protein 1, RBAP-1, Retinoblastoma-binding protein 3, RBBP-3, pRB-binding protein E2F-1

Gene Name

E2F1

Gene ID

1869

NCBI Gene ID

UniProt

Q01094

Accession Number

NP_005216

Cellular Locus

Nucleus

Host

Mouse

Reactivity

Human

Immunogen

GST fusion protein expressed in E. coli corresponding to aa 162-476 of human E2F-1.

Target Antigen

Transcription factor E2F1

Target

E2F-1

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, ICC/IF, IP, IHC

Field of Research

Cancer Research

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Transcription activator that binds DNA cooperatively with DP proteins through the E2 rPubMed:10675335, PubMed:12717439, PubMed:17050006, PubMed:17704056, PubMed:18625225, PubMed:20040599, PubMed:20176812, PubMed:28992046, PubMed:8170954}.

Additionnal Information

Immunoblotting: use at 1-5 ug/mL. <br><br>Immunoprecipitation: use at 1-5 ug/mL. <br><br>Positive controls: E2F-1 transfected Soas2 cells and recombinant fusion protein.