Biotinylated Rhesus macaque Mamu-A*08&B2M Monomer Protein (Peptide free, MALS verified)
Biotinylated Rhesus macaque Mamu-A*08&B2M Monomer Protein (MAM-R82E5) is expressed from human 293 cells (HEK293) . It contains AA Gly 17 - Pro 299 (Mamu-A*08) & Ile 21 - Met 119 (B2M) (Accession # Q9MW40 (Mamu-A*08) & Q6V7J5 (B2M) ) .
Product Specifications
Specifications
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 35.7 kDa and 11.6 kDa. The protein migrates as 40-42 kDa and 10 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
Q9MW40 (Mamu-A*08) & Q6V7J5 (B2M)
Expression Region
Gly 17 - Pro 299 (Mamu-A*08) & Ile 21 - Met 119 (B2M)
Host
HEK293
Target
Mamu-A*08 & B2M
Conjugation
Biotin-labeled
Tag
C-10xHis & C-Avi
Source
Rhesus macaque
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
95%
Bioactivity
Immobilized Biotinylated Rhesus macaque Mamu-A*08&B2M Monomer Protein (Cat. No. MAM-R82E5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-4 ng/mL (QC tested).
Format
Powder
Buffer
PBS, pH7.4
Additives
Trehalose
Molecular Weight
35.7 kDa and 11.6 kDa
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
25ug*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/MAM-R82E5.pdf
Species
Rhesus macaque
Curated Selection
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