Coelenterazine h

Coelenterazine h (2-Deoxycoelenterazine), a coelenterazine derivative, is a luminescent substrate for RLuc8. Coelenterazine h is more sensitive to Ca2+, thus providing a valuable tool for measuring small changes in Ca2+ concentrations (Ex/Em = 437/466 nm) [1][2][3][4].

Product Specifications

CAS Number

[50909-86-9]

Product Name Alternative

2-Deoxycoelenterazine; CLZN-h

UNSPSC

12171500

Hazard Statement

H302, H315, H319, H335

Target

Calcium Channel

Type

Dye Reagents

Related Pathways

Membrane Transporter/Ion Channel; Neuronal Signaling

Applications

Neuroscience-Neuromodulation

Field of Research

Neurological Disease

Assay Protocol

https://www.medchemexpress.com/Coelenterazine_h.html

Purity

99.41

Solubility

DMSO : 100 mg/mL (ultrasonic)

Smiles

OC(C=C1)=CC=C1C2=CN(C(C(CC3=CC=CC=C3)=N4)=O)C4=C(N2)CC5=CC=CC=C5

Molecular Formula

C26H21N3O2

Molecular Weight

407.46

Precautions

H302, H315, H319, H335

References & Citations

[1]Jiang T, et al. New bioluminescent coelenterazine derivatives with various C-6 substitutions. Org Biomol Chem. 2017 Aug 23;15 (33) :7008-7018.|[2]M R Knight, et al. Imaging calcium dynamics in living plants using semi-synthetic recombinant aequorins. J Cell Biol. 1993 Apr;121 (1) :83-90. |[3]Kazushi Suzuki, et al. Five colour variants of bright luminescent protein for real-time multicolour bioimaging. Nat Commun. 2016 Dec 14:7:13718. |[4]Kenta Saito, et al. Auto-luminescent genetically-encoded ratiometric indicator for real-time Ca2+ imaging at the single cell level. PLoS One. 2010 Apr 1;5 (4) :e9935.