Human GITR / TNFRSF18 Protein, Mouse IgG2a Fc Tag, low endotoxin

Human GITR Protein, Mouse IgG2a Fc Tag (GIR-H525a) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Glu 161 (Accession # Q9Y5U5-1).

Product Specifications

Background

Glucocorticoid-induced TNFR-related protein (GITR) is also known as Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), activation-inducible TNFR family receptor (AITR), CD antigen CD357, which is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is receptor for TNFSF18, which seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. GITR also mediated NF-kappa-B activation via the TRAF2/NIK pathway.

Specifications

This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 41.4 kDa. The protein migrates as 46-49 kDa and 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Gln 26 - Glu 161

Host

HEK 293

Target

GITR

Conjugation

Unconjugated

Tag

C-mFc(mIgG2a)

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.1 EU per μg

Purity

95%

Bioactivity

Immobilized Human GITR Ligand, Fc Tag (Cat. No. GIL-H526a) at 5 μg/mL (100 μL/well) can bind Human GITR Protein, Mouse IgG2a Fc Tag (Cat. No. GIR-H525a) with a linear range of 0.039-0.313 μg/mL (QC tested).