Endo S2/Endo-beta-N-acetylglucosaminidase
Endo S2 recognizes the conserved N-glycans on the Fc region of IgG by hydrolyzing the chitobiose core (at the beta -1,4 linkage between the two N-acetylglucosamines) of the N-glycans.
Product Specifications
Product Name Alternative
Endo S2, Endo-S2, EndoS2, Endo-beta-N-acetylglucosaminidase
Expression System
E.coli
Nature
Recombinant
Applications
Endo S2 hydrolyses N-linked glycans on IgG and α1-acid glycoprotein.
Stability
Endotoxin
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Bioactivity
Unit Definition - One unit deglycosylates ≥ 95% of 1 μg human IgG, when incubated in 10 mM sodium phosphate, 150 mM NaCl, pH7.4 at 37°C for 30 min.
Form
Lyophilized
Buffer
Lyophilized from PBS pH7.4,1mM EDTA, 4%trehalose,1% mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution.
Shipping Conditions
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Notes
For research use only.
Species
Streptococcus pyogenes
Frequently Asked Questions
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