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Endo S2/Endo-beta-N-acetylglucosaminidase

Endo S2 recognizes the conserved N-glycans on the Fc region of IgG by hydrolyzing the chitobiose core (at the beta -1,4 linkage between the two N-acetylglucosamines) of the N-glycans.

Product Specifications

Product Name Alternative

Endo S2, Endo-S2, EndoS2, Endo-beta-N-acetylglucosaminidase

Expression System

E.coli

Nature

Recombinant

Applications

Endo S2 hydrolyses N-linked glycans on IgG and α1-acid glycoprotein.

Stability

Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.

Endotoxin

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Bioactivity

Unit Definition - One unit deglycosylates ≥ 95% of 1 μg human IgG, when incubated in 10 mM sodium phosphate, 150 mM NaCl, pH7.4 at 37°C for 30 min.

Form

Lyophilized

Buffer

Lyophilized from PBS pH7.4,1mM EDTA, 4%trehalose,1% mannitol.

Reconstitution

Reconstitute in sterile water for a stock solution.

Shipping Conditions

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Notes

For research use only.

Species

Streptococcus pyogenes

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