AKT1/2/3 Recombinant Rabbit Monoclonal Antibody [JE75-09]
Akt, also referred to as PKB or Rac, plays a critical role in controlling cell survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 . Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad, forkhead transcription factors, c-Raf, and caspase-9. PTEN phosphatase is a major negative regulator of the PI3K/Akt signaling pathway. LY294002 is a specific PI3 kinase inhibitor. Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β. Akt may also play a role in insulin stimulation of glucose transport. In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 and by negatively regulating the cyclin-dependent kinase inhibitors p27 Kip1 and p21 Waf1/Cip1. Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor. More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex.
Product Specifications
Product Name Alternative
Abbreviation
AKT1, PKB, RAC, AKT2, AKT3, PKBG
Swiss Prot
P31749 Human, P31751 Human, Q9Y243 Human, P31750 Mouse, Q60823 Mouse, Q9WUA6 Mouse, P47196 Rat, P47197 Rat, Q63484 Rat
Cellular Locus
Cytoplasm, Nucleus, Cell membrane.
Host
Rabbit
Species Reactivity
Human,Mouse,Rat,Monkey
Immunogen
Recombinant protein within Human AKT1/2/3 aa 231-480 / 480.
Isotype
IgG
Clone
JE75-09
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Cell, FC, IP
Positive Control
Concentration
1 mg/mL
Dilution
WB: 1:2,000 ;IF-Cell: 1:100 ;FC: 1:1,000 ;IP: 1-2μg/sample
Purity
Protein A affinity purified.
Form
Liquid
Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 56 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
Yes
CAS Number
9000-83-3
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