Anti-ILP-2 Antibody

Rabbit Polyclonal Antibody specific to ILP-2

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Inhibitor of apoptosis-like protein 2, IAP-like protein 2, ILP-2, Testis-specific inhibitor of apoptosis

Gene Name

BIRC8

Gene ID

112401

NCBI Gene ID

UniProt

Q96P09

Accession Number

NP_203127

Cellular Locus

Cytoplasm

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Peptide corresponding to aa 2-13 of human ILP-2 (accession no. NP_203127).

Target Antigen

Baculoviral IAP repeat-containing protein 8

Target

ILP-2

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, ICC, IF

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Protects against apoptosis mediated by BAX.

Additionnal Information

Immunoblotting: use at 1ug/mL. Immunocytochemistry: use at 10ug/mL. Positive control: HepG2 or MOLT4 cell lysates. These are recommended concentrations. Enduser should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human, mouse, and rat ILP-2 (33kDa).

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family including IAP, XIAP/ILP-1/MIHA, and Livin/KIAP that bind to and inhibit specific proteases. A novel member in the IAP protein family has been identified and designated ILP-2 for IAP-like protein-2 (1). ILP-2 has high homology to ILP-1, but is encoded by a distinct gene. ILP-2, unlike ILP-1, has no inhibitory effect on Fas and TNF induced apoptosis, but potently inhibits apoptosis induced by overexpression of Bax or by coexpression of caspase-9 with Apaf-1. ILP-2 interacts with the processed caspase- 9.