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ApoFlowEx FITC Kit

The ApoFlowEx FITC Kit is intended for differentiation of early apoptotic, necrotic and viable cells.

Product Specifications

Certification

RUO

Applications

FC (QC tested)

Components

ED7044-1 Annexin V-FITC – 1 vial, 0.5 mL of Ready-to-Use solution. No reconstitution is necessary. Reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide. Sufficient for 100 tests. ED7044-2 Propidium Iodide – 1 vial, 0.5 mL of Ready-to-Use solution in deionized water (0.1 mg/mL) . Sufficient for 100 tests. ED7044-3 Annexin V Binding Buffer (10x) – 1 vial, 5 ml of 10x concentrated filter-sterilized solution (0.1M HEPES/NaOH, pH 7.4, 1.4M NaCl, 25mM CaCl) . Dilute 10x in deionized water prior to use.

References & Citations

*Drápela S, Khirsariya P, van Weerden WM, Fedr R, Suchánková T, Búzová D, Červený D, Hampl A, Puhr M, Watson WR, Culig Z, Krejčí L, Paruch K, Souček K: The CHK1 inhibitor MU380 significantly increases the sensitivity of human docetaxel‐resistant prostate cancer cells to gemcitabine through the induction of mitotic catastrophe. Molecular Oncology 14, Issue 10., URL: https://febs.onlinelibrary.wiley.com/doi/10.1002/1878-0261.12756, *Vahidi R, Safi S, Farsinejad A, Panahi N: Citrate and celecoxib induce apoptosis and decrease necrosis in synergistic manner in canine mammary tumor cells. Cell. Mol. Biol. 2015, 61 (5) : 22-28., URL: https://pubmed.ncbi.nlm.nih.gov/26475384/, *Pavliukeviciene B, Zentelyte A, Jankunec M, Valiuliene G, Talaikis M, Navakauskiene R, Niaura G, Valincius G: Amyloid β oligomers inhibit growth of human cancer cells. PLoS One. 2019 Sep 11;14 (9) :e0221563., URL: https://pubmed.ncbi.nlm.nih.gov/31509551/, *Rahimifard M, Baeeri M, Bahadar H, Moini-Nodeh S, Khalid M, Haghi-Aminjan H, Mohammadian H, Abdollahi M: Therapeutic Effects of Gallic Acid in Regulating Senescence and Diabetes; an In Vitro Study. Molecules 2020 Dec 11;25 (24) :5875. , URL: https://www.mdpi.com/1420-3049/25/24/5875/htm, *Rahmani A, Mousavi HZ, Salehi R, Bagheria A: Novel pH-sensitive and biodegradable micelles for the combined delivery of doxorubicin and conferone to induce apoptosis in MDA-MB-231 breast cancer cell line. RSC Advances 2020, Issue 49, URL: https://pubs.rsc.org/en/content/articlelanding/2020/ra/d0ra03467c#!divAbstract, *Stefanache T, Forna N, Badescu M, Jitaru D, Dragos ML, Rezus C, Diaconescu BM, Badulescu O, Rezus E, Ciocoiu M, Badescu C: Modulation of the activity of certain genes involved in tumor cell metabolism in the presence of the cytotoxic peptides defensin and cathelicidin LL37. Exp Ther Med. 2019 Dec;18 (6) :5033-5040., URL: https://www.spandidos-publications.com/10.3892/etm.2019.8117, *Krátká Z, Luxová Š, Vik V: Flow cytometry analysis of sperm quality in sub-fertile men. Ces Urol 2017; 21 (3) : 277-286, URL: https://www.prolekare.cz/casopisy/ceska-urologie/2017-4/vyuziti-prutokove-cytometrie-pro-stanoveni-kvality-ejakulatu-u-muzu-s-poruchou-reprodukce-63057, *Doustvandi MA, Mohammadnejad F, Mansoori B, Tajalli H, Mohammadi A, Mokhtarzadeh A, Baghbani E, Khaze V, Hajiasgharzadeh K, Moghaddam MM, Hamblin MR, Baradaran B: Photodynamic therapy using zinc phthalocyanine with low dose of diode laser combined with doxorubicin is a synergistic combination therapy for human SK-MEL-3 melanoma cells. Photodiagnosis Photodyn Ther. 2019 Dec;28:88-97. , URL: https://pubmed.ncbi.nlm.nih.gov/31454716/, *Pedram S, Mohammadirad A, Rezvanfar MA, Navaei-Nigjeh M, Baeeri M, Abdollahi M: On the protection by the combination of CeO2 nanoparticles and sodium selenite on human lymphocytes against chlorpyrifos-induced apoptosis in vitro. CELL JOURNAL (Yakhteh), 2015, 17, 2, 361-371., URL: https://applications.emro.who.int/imemrf/Cell_J_Yakhteh/Cell_J_[Yakhteh]_2015_17_2_361_371.pdf, *Payandeh Z, Tazehkand AP, Mansoori B, Khaze V, Samadi N, Baradaran B: The potential role of Nrf2-PD-L1 axis in promoting of oxaliplatin resistance in colon cancer cells. doi.org/10.21203/rs.3.rs-58926/v1, URL: https://assets.researchsquare.com/files/rs-58926/v1/a829c994-504f-491d-853a-1425326ce526.pdf, *Lasta CS, Hlavac N, Marcondes NA, Dalmolin ML, Terra SR, de Almeida Lacerda L, Faulhaber GA, González FH: Quality control in veterinary blood banks: evaluation of canine platelet concentrates stored for five days. BMC Veterinary Res. 2020, 16:25, URL: https://link.springer.com/article/10.1186/s12917-020-2254-5, *Ghaemmaleki F, Mohammadi P, Baeeri M, Navaei-Nigjeh M, Abdollahi M, Mostafalou S: Estrogens counteract tributyltin-induced toxicity in the rat islets of Langerhans. Heliyon 2020, 6, 3, e03562, URL: https://www.sciencedirect.com/science/article/pii/S2405844020304072, *Haghi-Aminjan H, Baeerib M, Rahimifard M, Alizadeh A, Hodjat M, Hassani S, Asghari MH, Abdollahi A, Didari T, Hosseini R, Sharifzadeh M, Abdollahi M: The role of minocycline in alleviating aluminum phosphide-induced cardiac hemodynamic and renal toxicity. Env. Toxicol. Pharmacol. 64 (2018) 26-40., URL: https://daneshyari.com/article/preview/11023027.pdf

Storage Conditions

Store at 2-8°C. Do not freeze.

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