Anti-BRCAA1 Antibody

ARID domain-containing protein 4B, 180 kDa Sin3-associated polypeptide, Sin3-associated polypeptide p180, Breast cancer-associated antigen BRCAA1, Histone deacetylase complex subunit SAP180, Retinoblastoma-binding protein 1-like 1

Acts as a transcriptional repressor (PubMed:12724404). May function in the assembly and/or enzymatic activity of the Sin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes (PubMed:12724404). Plays a role in the regulation of epigenetic modifications at the PWS/AS imprinting center near the SNRPN promoter, where it might function as part of a complex with RB1 and ARID4A. Involved in spermatogenesis, together with ARID4A, where it functions as a transcriptional coactivator for AR (androgen receptor) and enhances expression of genes required for sperm maturation. Regulates expression of the tight junction protein CLDN3 in the testis, which is important for integrity of the blood-testis barrier. Plays a role in myeloid homeostasis where it regulates the histone methylation state of bone marrow cells and expression of various genes involved in hematopoiesis. May function as a leukemia suppressor (By similarity). {UniProtKB:A2CG63, PubMed:12724404}.

Immunoblotting : use at 1:500-1:1,000 dilution. A band of approximately 136 kDa is detected. <br><br>Positive control: MCF-7 human breast cancer cell lysate.

The novel peptide sequence SSKKQKRSHK within BRCAA1 protein was mapped to the cytoplasm of MCF-7 cells. Immunohistochemical analysis demonstrated that this sequence was expressed in 65% of breast tumors. Its expression may be useful in diagnosis and prognosis of breast cancer.