What is the catalog number for Anti-BRCAA1 Antibody?

The catalog number for Anti-BRCAA1 Antibody is 34022-1.

What sizes are available for Anti-BRCAA1 Antibody?

Anti-BRCAA1 Antibody is available in the following size(s): 100 µg.

Is Anti-BRCAA1 Antibody in stock?

Anti-BRCAA1 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

What is the purity of this recombinant protein?

Purity, typically assessed by SDS-PAGE, is listed on the datasheet under quality control data.

What do I do if this product arrives with reduced ice or a damaged cold-chain package?

Report this to us immediately with photos and your order reference. Our specialists will assess whether the product is still usable given the cold-chain interruption, and will arrange a replacement or credit if it isn’t.

What host species was used to raise this antibody?

The host species used in production is listed under product specifications on the datasheet, and is relevant when selecting a compatible secondary antibody for your assay.

Anti-BRCAA1 Antibody

Rabbit Polyclonal Antibody specific to BRCAA1

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

ARID domain-containing protein 4B, 180 kDa Sin3-associated polypeptide, Sin3-associated polypeptide p180, Breast cancer-associated antigen BRCAA1, Histone deacetylase complex subunit SAP180, Retinoblastoma-binding protein 1-like 1

Gene Name

ARID4B

NCBI Gene ID

UniProt

Q4LE39

Cellular Locus

Nucleus, Cytoplasm

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acids 1046- 1055 (SSKKQKRSHK) of human BRCAA1 conjugated to KLH.

Target Antigen

AT-rich interactive domain-containing protein 4B

Target

BRCAA1

Clonality

Polyclonal

Type

Antibody

Applications

Field of Research

Cancer research

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Acts as a transcriptional repressor (PubMed:12724404). May function in the assembly and/or enzymatic activity of the Sin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes (PubMed:12724404). Plays a role in the regulation of epigenetic modifications at the PWS/AS imprinting center near the SNRPN promoter, where it might function as part of a complex with RB1 and ARID4A. Involved in spermatogenesis, together with ARID4A, where it functions as a transcriptional coactivator for AR (androgen receptor) and enhances expression of genes required for sperm maturation. Regulates expression of the tight junction protein CLDN3 in the testis, which is important for integrity of the blood-testis barrier. Plays a role in myeloid homeostasis where it regulates the histone methylation state of bone marrow cells and expression of various genes involved in hematopoiesis. May function as a leukemia suppressor (By similarity). {UniProtKB:A2CG63, PubMed:12724404}.

Additionnal Information

Immunoblotting : use at 1:500-1:1,000 dilution. A band of approximately 136 kDa is detected. <br><br>Positive control: MCF-7 human breast cancer cell lysate.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody recognizes human, mouse, and rat BRCAA1.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

The novel peptide sequence SSKKQKRSHK within BRCAA1 protein was mapped to the cytoplasm of MCF-7 cells. Immunohistochemical analysis demonstrated that this sequence was expressed in 65% of breast tumors. Its expression may be useful in diagnosis and prognosis of breast cancer.