QuicKey Pro Mouse IL-18 (Interleukin 18) ELISA Kit
Product Specifications
Abbreviation
IL-18
UniProt
P70380
Cross Reactivity
This kit recognizes IL-18 in samples.No significant cross-reactivity or interference between IL-18 and analogues was observed.
Target
IL18; IGIF; IL-1g; IL-1F4; Interferon-gamma-inducing factor
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Metabolism; Immunology
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-18. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse IL-18 are added to the micro ELISA plate wells. Mouse IL-18 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse IL-18.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-18. The concentration of Mouse IL-18 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
1 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.16-10 ng/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
0.09 ng/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,6 months
Detection Instrument
Microplate reader
Species
Mouse
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Mouse IL-18 concentrations in serum, plasma and other biological fluids.
Available Sizes
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