Mouse anti Human IgE (class specific), conjugated with Biotin

The reactivity of the antiserum is restricted to an isotype specific determinant of the IgE molecule as tested in direct binding enzyme immunoassay, immunoblotting, immuno- precipitation and direct immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgE in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgE, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.

Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline (pH7,2); Nopreservative added, as it may interfere with the antibody activity; Noforeign protein added

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C; Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product Diluted solutions should be stored at +4ºC, not refrozen, and preferably used the same day