Anti-c-Jun (Phospho-Ser243) Antibody

Rabbit Polyclonal Antibody specific to c-Jun (Phospho-Ser243)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-c-Jun (Phospho-Ser243) Rabbit Polyclonal Antibody

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Peptide sequence that includes phosphorylation site of Serine 243 (P-L-S(p)-P-I) derived from human c-Jun and conjugated to KLH.

Target

c-Jun (Phospho-Ser243)

Clonality

Polyclonal

Type

Antibody

Applications

WB, IHC

Field of Research

Phosphospecific Antibodies

Purification Method

Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~43kDa is detected. Immunohistochemistry: use at dilution of 1:50- 1:100. These are recommended working dilutions. End user should determine optimal dilutions for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody detects endogenous human, mouse, and rat c-Jun only when phosphorylated at serine 243.

Formulation

PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Buffer pH

pH 7.4

Target Background

c-Jun in combination with c-Fos, forms the AP-1 early response transcription factor. It was first identified as the Fos-binding protein p39 and only later rediscovered as the product of the c-jun gene. It is activated through double phosphorylation by the JNK pathway but has also a phosphorylation-independent function