CD74 Antibody

It recognizes a protein of ~35kDa, identified as CD74 (Workshop IV) . CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+) .

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-3 µg/mL

UniProt

P04233

Host

Mouse

Reactivity

Human

Immunogen

A recombinant fragment from the human protein was used as the immunogen for the CD74 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CLIP/7193

Applications

WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the CD74 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CD74 antibody is available for research use only.

Storage Conditions

Aliquot the CD74 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the CD74 antibody should be determined by the researcher.

Location

Cell surface, paranuclear globular

Image Legend

IHC staining of FFPE human spleen tissue with CD74 antibody (clone CLIP/7193) at 2ug/ml. Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.