CD74 Antibody
It recognizes a protein of ~35kDa, identified as CD74 (Workshop IV) . CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+) .
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-3 µg/mL
UniProt
P04233
Host
Mouse
Reactivity
Human
Immunogen
A recombinant fragment from the human protein was used as the immunogen for the CD74 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
CLIP/7193
Applications
WB, IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the CD74 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This CD74 antibody is available for research use only.
Storage Conditions
Aliquot the CD74 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the CD74 antibody should be determined by the researcher.
Location
Cell surface, paranuclear globular
Image Legend
Frequently Asked Questions
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