Keratin 14 Antibody / Cytokeratin 14 / KRT14

Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic) . CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P02533

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 351-472 from the human KRT14 protein was used as the immunogen for the Keratin 14 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

KRT14/4127

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the Keratin 14 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Keratin 14 antibody is available for research use only.

Storage Conditions

Aliquot the Keratin 14 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the Keratin 14 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

Analysis of HuProt (TM) microarray containing more than 19,000 full-length human proteins using Keratin 14 antibody (clone KRT14/4127) . These results demonstrate the foremost specificity of the KRT14/4127 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.