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S100A4 Antibody

S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, 4 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

Product Specifications

Specifications

ELISA: order antibody without BSA for coating, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P26447

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 1-200 from the human S100A4 protein was used as the immunogen for the S100A4 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

S100A4/6803

Applications

ELISA, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the S100A4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This S100A4 antibody is available for research use only.

Storage Conditions

Aliquot the S100A4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.nsjbio.com/tds-pdf/s100a4-antibody-s100a46803-v4048

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the S100A4 antibody should be determined by the researcher.

CAS Number

9007-83-4

Location

Cytoplasm, nucleus

Image Legend

IHC staining of FFPE human colon tissue with S100A4 antibody (clone S100A4/6803) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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