CD276 Antibody / B7-H3

T cell activation and immune function are regulated by the innate immune system through positive and negative costimulatory molecules. One such molecule, B7-H3 (B7-homolog 3, also designated B7RP-2 and CD276) belongs to the B7 immunoglobulin superfamily. Soluble B7-H3/CD276 binds a putative receptor on activated T-cells that is distinct from CD28, CTLA-4, ICOS and PD-1. Widely expressed on nonlymphoid tissues, it costimulates proliferation of both CD4+ and CD8+ T cells. The ability of B7-H3/CD276 to stimulate Th1 and cytotoxic-T cell responses suggest that it may have antitumor activity. B7-H3 interactions may play a role in regulating cell-mediated immune responses against cancer, implicating B7-H3/CD276 as a potential therapeutic tool.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q5ZPR3

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 100-300 from the human CD276 protein was used as the immunogen for the CD276 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

B7H3/4345

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the CD276 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CD276 antibody is available for research use only.

Storage Conditions

Aliquot the CD276 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the CD276 antibody should be determined by the researcher.

Location

Cell surface, cytoplasm

Image Legend

IHC staining of FFPE human ovarian carcinoma tissue with B7-H3 antibody (clone B7H3/4345) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.