SCFR Antibody / c-Kit / CD117

C-Kit, also called CD117, SCFR and Stem Cell Factor Receptor is found on a wide variety of tumor cells including follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, and breast as well as malignant melanoma, endodermal sinus tumor, and small cell carcinoma. However, anti-SCFR has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposi s sarcoma, tumors of smooth muscle origin, fibromatosis, and neural tumors of the GI tract. Anti-SCFR is also useful in recognizing myeloblasts in bone marrow biopsy and clot section.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: 2-4 µg/mL (order Ab without BSA for coating), Immunohistochemistry (FFPE) : 2-4 µg/mL

UniProt

P10721

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 50-250 from the human protein was used as the immunogen for the SCFR antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

C117/7119

Applications

ELISA, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the SCFR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This SCFR antibody is available for research use only.

Storage Conditions

Aliquot the SCFR antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the SCFR antibody should be determined by the researcher.

Location

Cell surface, cytoplasm

Image Legend

IHC staining of FFPE human gastrointestinal stromal tumor (GIST) with CD117 / SCFR antibody (clone C117/7119) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.