SUB1 Antibody / Positive cofactor 4 / PC4
Activated RNA polymerase II transcriptional coactivator p15, also known as positive cofactor 4 (PC4) or SUB1 homolog, is a protein that in humans is encoded by the SUB1 gene. This gene is mapped to 5p13.3. The transcriptional cofactor PC4 is an ancient single-strand DNA (ssDNA) -binding protein that has a homologue in bacteriophage T5 where it is likely the elusive replicative ssDNA-binding protein. The recombinant PC4 is shown to function identically to the native protein through its interaction with TAFs.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL
UniProt
P53999
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant human protein (amino acids N62-L127) was used as the immunogen for the SUB1 antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
6B5B10
Applications
WB, IHC-P
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
Limitations
This SUB1 antibody is available for research use only.
Storage Conditions
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the SUB1 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
IHC staining of FFPE human lung cancer tissue with SUB1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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