PDIA6 Antibody / ERp5

This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, two catalytically active thioredoxin (TRX) domains, a TRX-like domain, and a C-terminal ER-retention sequence. This protein inhibits the aggregation of misfolded proteins and exhibits both isomerase and chaperone activity. Alternative splicing results in multiple transcript variants encoding different isoforms.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

Q15084

Host

Mouse

Reactivity

Human, Monkey

Immunogen

Recombinant human protein (amino acids L20-L440) was used as the immunogen for the PDIA6 antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

3H5E7

Applications

WB, IHC-P, IF, FACS

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the PDIA6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This PDIA6 antibody is available for research use only.

Storage Conditions

After reconstitution, the PDIA6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the PDIA6 antibody should be determined by the researcher.

Location

Cytoplasmic, cell membrane

Image Legend

IHC staining of FFPE human breast infiltrating ductal carcinoma tissue with PDIA6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.