CD20 Antibody

CD20, also known as MS4A1, is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. It is mapped to 11q12.2. This gene encodes a member of the membrane-spanning 4A gene family. The function of CD20 is to enable optimal B-cell immune response, specifically against T-independent antigens. It is suspected that CD20 acts as a calcium channel in the cell membrane. Whatï¿½s more, this protein may be involved in the regulation of B-cell activation and proliferation.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL

UniProt

P11836

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human CD20 protein (amino acids M1-D261) was used as the immunogen for the CD20 antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

4D11

Applications

WB, IHC-P, IF

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the CD20 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This CD20 antibody is available for research use only.

Storage Conditions

After reconstitution, the CD20 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the CD20 antibody should be determined by the researcher.

Location

Predominantly cell surface with some cytoplasmic

Image Legend

Immunofluorescent staining of FFPE human tonsil tissue with CD20 antibody (green), CD3e antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH8 EDTA buffer for 20 min.